《植物生理学报》 2013, 49(3): 247-252

花生开花下针期¹⁴C-生长素的运输与分布

彭琼, 李合松, 张顺超, Mohammed H. Kabir, 黄志刚, 萧浪涛*

湖南农业大学植物激素与生长发育湖南省重点实验室, 长沙410128

通信作者：萧浪涛；E-mail: langtaoxiao@163.com；Tel: 0731-84635261

摘　要：

为研究花生开花下针期生长素在植株中的运输与分布规律, 以‘中花4号’花生为试验材料, 以14C-IAA为示踪物, 对花生不同部位中14C-IAA的分布进行放射自显影定位分析, 并利用液体闪烁计数仪进行定量检测。结果表明: 涂布于花生主茎顶叶的14C-IAA在处理48 h后向根和茎中的转运量增加, 向下运输的速率约为5 mm∙h-1。通过浸泡处理进入根系的14CIAA具有向顶传导的能力, 但在根系中的滞留量较高, 处理24 h时14C-IAA向地上部分转运的能力较强。涂布于果针着生部位上方茎段的14C-IAA可同时向上、下两个方向运输, 且处理24 h时运输能力较强。带有果针的花在涂布处理72 h后其14CIAA主要向下运输; 而尚未形成果针的花涂布处理后其14C-IAA运输至不同部位的含量为: 叶>茎>根。

关键词：同位素示踪; 14C-IAA; 生长素运输; 开花下针期

收稿：2013-01-11   修定：2013-01-25

资助：国家自然科学基金重大研究计划项目(90817101和9111-7006)和湖南农业大学2011年湖南省研究生科研创新项目(CX2011B291)。

Transportation and Distribution of ¹⁴C-Labelled Auxin in the Pegging Stage of Peanut (Arachis hypogaea L.)

PENG Qiong, LI He-Song, ZHANG Shun-Chao, Mohammed H. Kabir, HUANG Zhi-Gang, XIAO Lang-Tao*

Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China

Corresponding author: XIAO Lang-Tao; E-mail: langtaoxiao@163.com; Tel: 0731-84635261

Abstract:

In order to reveal the transportation and distribution of ¹⁴C-labelled auxin in different parts at the pegging stage of peanut, ‘Zhonghua 4’ was used as the experimental material by locally applying ¹⁴C-IAA on top leaves, roots, flowers and stem portions below where pegs were originated, the autoradiographs were analysed and the radioactivity in the different parts was determined by using a liquid scintillation counter. The results showed that the ¹⁴C-IAA contents in roots and stems were higher than in leaves at 48 h after the treatment, and the transport rate was approximately 5 mm∙h-1 from top leaves to downwards of stems when top leaves were treated. In case of root treatment, ¹⁴C-IAA had a transportation trend upwards. The accumulation of ¹⁴C-IAA in roots was higher than in other parts, moreover the upwards transportation ability of ¹⁴C-IAA was stronger at 24 h after the treatment. Whereas ¹⁴C-IAA applied in the stem portions transported both upwards and downwards simultaneously, and ¹⁴C-IAA content was found to be high in roots, stems and leaves after treated 24 h. The transportation of ¹⁴C-IAA from flowers with pegs was found mainly downwards after treated for 72 h. However the highest content of ¹⁴C-IAA was found in leaves followed by stems and the lowest was in roots in case of ¹⁴C-IAA transportation from flowers without pegs.

Key words: isotopic tracing; ¹⁴C-IAA; auxin transport; pegging stage